Fermented Sugar From Ultrasound-Assisted Acid Hydrolysis Berenuk Fruit (Crecentia Cujete L.)

Nita Indriyani, Lusiana Lumbantoruan, Ummu Musfika, F. Fathurrahman

Abstract

Berenuk fruit (Crecentia Cujete L) is a non-food plant often found in tropical areas. Berenuk has quite a high content of carbohydrates and its derivatives. Among them are 18.61% carbohydrates, 59.86% sucrose, 25.09% fructose, and 18.24% galactose, so berenuk fruit has the potential to be a source or ingredient for bioethanol. The bioethanol process from biomass goes through several steps: raw material preparation, hydrolysis process and fermentation. The hydrolysis process is the process of breaking down carbohydrate into glucose units which are connected by β-1,4 glycosidic bonds. Another method of breaking sugar polymers can be done using alternative energy such as ultrasonic waves (50Hz, 50W) or microwaves. In this research, an ultrasonic-assisted acid hydrolysis process was carried out. The type of acid used is concentrated sulfuric acid (H2SO4). Ingredients ratio 1:20 (w/v) berenuk powder sample with sulfuric acid. The sulfuric acid concentrations used were 1 M, 2 M, and 3 M. Hydrolysis times were 40 minutes, 60 minutes, and 80 minutes at a temperature of 1000C. The results of research that has been carried out on the preparation process of bereneuk fruit have an average water content of 97.44%, so treatment is required before proceeding to the hydrolysis and fermentation process, namely by drying the drying process is carried out directly in sunlight for seven days, then continued by heating the oven for 8 hours at 1000C. In the ultrasonic wave assisted hydrolysis process carried out at a H2SO4 concentration of 3 M, a hydrolysis time of 80 minutes, and a temperature of 1000C produces 22.20% glucose.

Keywords

bioethanol; hydrolysis; fermentation; sugar reduction

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References

O. A. Fakayode dkk., “Simplistic two-step fabrication of porous carbon-based biomass-derived electrocatalyst for efficient hydrogen evolution reaction,” Energy Conversion and Management, vol. 227, hlm. 113628, Jan 2021, doi: 10.1016/j.enconman.2020.113628.

T. Suganya, M. Varman, H. H. Masjuki, dan S. Renganathan, “Macroalgae and microalgae as a potential source for commercial applications along with biofuels production: a biorefinery approach,” Renewable and Sustainable Energy Reviews, vol. 55, hlm. 909–941, 2016.

O. A. Fakayode, E. A. A. Aboagarib, C. Zhou, dan H. Ma, “Co-pyrolysis of lignocellulosic and macroalgae biomasses for the production of biochar–a review,” Bioresource technology, vol. 297, hlm. 122408, 2020.

M. Karimi, B. Jenkins, dan P. Stroeve, “Ultrasound irradiation in the production of ethanol from biomass,” Renewable and Sustainable Energy Reviews, vol. 40, hlm. 400–421, Des 2014, doi: 10.1016/j.rser.2014.07.151.

B. C. Ejelonu, A. A. Lasisi, A. G. Olaremu, dan O. C. Ejelonu, “The chemical constituents of calabash (Crescentia cujete),” African Journal of Biotechnology, vol. 10, no. 84, hlm. 19631–19636, 2011.

N. Arviana, L. Aini, R. R. Yogaswara, dan E. A. Saputro, “Konversi Kulit Petai Menjadi Glukosa Melalui Hidrolisa Enzimatik Menggunakan Enzim Amilase,” 1, vol. 17, hlm. 96–100, Agu 2021.

A. Sasongko dan B. Gotama, “Hidrolisis Limbah Kulit Nanas dengan Asam Asetat Menggunakan Metode Ultrasound-Assisted Acid Hydrolysis (UAAH) untuk Produksi Oligosakarida,” JST (Jurnal Sains Terapan), vol. 5, no. 2, Art. no. 2, 2019, doi: 10.32487/jst.v5i2.747.

D. Santos, U. F. Silva, F. A. Duarte, C. A. Bizzi, E. M. M. Flores, dan P. A. Mello, “Ultrasound-assisted acid hydrolysis of cellulose to chemical building blocks: Application to furfural synthesis,” Ultrasonics Sonochemistry, vol. 40, hlm. 81–88, Jan 2018, doi: 10.1016/j.ultsonch.2017.04.034.

L. Fitriana, M. Hidayah, dan W. Astuti, “Sintesis Nanoselulosa dari Batang Bambu menggunakan Hidrolisis Asam dan Gelombang Ultrasonik sebagai adsorben Logam Kadmium(II) dalam Limbah Industri Elektroplating,” Prosiding SNTK Eco-SMART, vol. 1, no. 1, Art. no. 1, Jan 2019, Diakses: 25 Maret 2023. [Daring]. Tersedia pada: https://jurnal.uns.ac.id/ecosmart/article/view/29610

R. Ratnawati dan N. Indriyani, “Kinetics and Thermodynamics Study of Ultrasound-Assisted Depolymerization of k-Carrageenan in Acidic Solution,” Bull. Chem. React. Eng. Catal., vol. 15, no. 1, hlm. 280–289, Apr 2020, doi: 10.9767/bcrec.15.1.6738.280-289.

N. Indriyani, Y. L. Goa, M. Ely, dan E. Mendy, “Ultrasonic-Assisted Flavonoid Extraction from Ant Nest,” CHEESA: Chemical Engineering Research Articles, vol. 5, no. 2, Art. no. 2, Sep 2022, doi: 10.25273/cheesa.v5i2.12231.59-64.

L. B. Werle dkk., “Ultrasound-assisted acid hydrolysis of palm leaves (Roystonea oleracea) for production of fermentable sugars,” Industrial Crops and Products, vol. 45, hlm. 128–132, 2013.

E. Cako, Z. Wang, R. Castro-Muñoz, M. P. Rayaroth, dan G. Boczkaj, “Cavitation based cleaner technologies for biodiesel production and processing of hydrocarbon streams: A perspective on key fundamentals, missing process data and economic feasibility – A review,” Ultrasonics Sonochemistry, vol. 88, hlm. 106081, Agu 2022, doi: 10.1016/j.ultsonch.2022.106081.

Bahroni, “Pemanfaatan Buah Berenuk (Crescentia cujete Linn) Sebagai Bahan Baku Pembuatan Bioetanol,” INA-Rxiv, preprint, Jun 2018. doi: 10.31227/osf.io/2kxcv.

M. Ichwan, I. A. Dewi, dan Z. M. S, “Klasifikasi Support Vector Machine (SVM) Untuk Menentukan TingkatKemanisan Mangga Berdasarkan Fitur Warna,” MIND (Multimedia Artificial Intelligent Networking Database) Journal, vol. 3, no. 2, Art. no. 2, 2018.

E. Martins, D. Poncelet, dan D. Renard, “A novel method of oil encapsulation in core-shell alginate microcapsules by dispersion-inverse gelation technique,” Reactive and Functional Polymers, vol. 114, hlm. 49–57, Mei 2017, doi: 10.1016/j.reactfunctpolym.2017.03.006.

M. P. M. Tambunan, Z. Ginting, R. Nurlaila, M. Muhammad, dan I. Ishak, “Pengaruh Suhu Dan Waktu Hidrolisis Terhadap Kadar Glukosa Dalam Pembuatan Sirup Glukosa Dari Biji Alpukat Dengan Metode Hidrolisis Asam,” Chemical Engineering Journal Storage (CEJS), vol. 1, no. 3, hlm. 17–26, 2021.

S. Gok, M. Severcan, E. Goormaghtigh, I. Kandemir, dan F. Severcan, “Differentiation of Anatolian honey samples from different botanical origins by ATR-FTIR spectroscopy using multivariate analysis,” Food Chemistry, vol. 170, hlm. 234–240, Mar 2015, doi: 10.1016/j.foodchem.2014.08.040.

M. F. Cengiz dan M. Z. Durak, “Rapid detection of sucrose adulteration in honey using Fourier transform infrared spectroscopy,” Spectroscopy Letters, vol. 52, no. 5, hlm. 267–273, Mei 2019, doi: 10.1080/00387010.2019.1615957.

Iriany, H. Angkasa, dan C. A. Namira, “Ekstraksi Tanin dari Buah Balakka (Phyllanthus emblica L.) dengan Bantuan Microwave: Pengaruh Daya Microwave, Perbandingan Massa Kering Terhadap Jumlah Pelarut Etil Asetat,” J. Teknik Kimia, vol. 10, no. 1, hlm. 8–12, Mar 2021, doi: 10.32734/jtk.v10i1.5318.

Y. Yustinah, U. H. Hasyim, S. Ab, dan A. Aliyah, “Pengaruh Konsentrasi Asam Sulfat Pada Proses Hidrolisis Dedak Padi Menjadi Glukosa Untuk Pembuatan Plastik Biodegradabel,” Prosiding Semnastek, vol. 0, no. 0, Art. no. 0, Nov 2018, Diakses: 19 Maret 2023. [Daring]. Tersedia pada: https://jurnal.umj.ac.id/index.php/semnastek/article/view/3587

E. K. Artati, F. I. W. H, dan F. Fatimah, “Pengaruh Jenis Dan Konsentrasi Asam Terhadap Kinetika Reaksi Hidrolisis Pelepah Pisang (Musa Paradisiaca L),” Ekuilibrium, vol. 11, no. 2, Art. no. 2, Mar 2021.

M. S. Fajri, M. A. Satrio, L. I. Utami, dan K. N. Wahyusi, “Produksi Gula Cair dengan Proses Hidrolisis Asam dengan Bahan Pati Singkong,” ChemPro, vol. 3, no. 1, Art. no. 1, Mar 2022, doi: 10.33005/chempro.v3i1.157.

S. M. D. Kolo, J. Presson, dan P. Amfotis, “Produksi Bioetanol sebagai Energi Terbarukan dari Rumput Laut Ulva reticulata Asal Pulau Timor,” ALCHEMY J.Pen.Kim, vol. 17, no. 2, hlm. 159, Sep 2021, doi: 10.20961/alchemy.17.2.45476.159-167.

S. M. D. Kolo, “The Effect of Hydrolysis Time Using Microwave on Bioethanol Production from Sorghum Waste (Sorghum Bicolor L.),” JSTK, vol. 16, no. 1, hlm. 28, Mar 2022, doi: 10.20527/jstk.v16i1.11404.

S. R. Gusmarwani, M. S. P. Budi, W. B. Sediawan, dan M. Hidayat, “Pengaruh perbandingan berat padatan dan waktu reaksi terhadap gula pereduksi terbentuk pada hidrolisis bonggol pisang,” Jurnal Teknik Kimia Indonesia, vol. 9, no. 3, Art. no. 3, Okt 2018.

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