Uji Aktivitas Antibakteri Ekstra Virgin Olive Oil (EVOO) Terhadap Streptococcus Agalactiae

Wenny Rahmawati, Dwi Norma Retnaningrum


Streptococcus agalactiae is a pathogen known to frequently cause infections in both mothers and children. It can lead to complications such as premature birth, premature rupture of membranes, postpartum infections, pneumonia, meningitis, and sepsis. Current treatment for Streptococcus agalactiae often involves antibiotics, which can contribute to antibiotic resistance. Given the increasing concern about antibiotic resistance, the development of herbal antibacterials is gaining momentum, with extra virgin olive oil (EVOO) being one such area of interest. EVOO contains oleuropein, flavonoids, and essential oils, all of which possess antibacterial properties. The objective of this research is to demonstrate the antibacterial activity of extra virgin olive oil (EVOO). The study employed a true experimental design with a post-test-only control group, using the disc diffusion method to measure bacterial inhibition zones. The results revealed the largest inhibition zone, measuring 15.9 mm, with a 3 ml dose of EVOO. The correlation test indicated a significant value of 0.000, with a coefficient of -726, suggesting that an increase in EVOO dose results in a reduction in the Streptococcus agalactiae bacterial population. Based on these findings, it can be concluded that extra virgin olive oil (EVOO) exhibits potent antibacterial activity against Streptococcus agalactiae.



Streptococcus agalactiae; Extra virgin olive oil; Antibacterial

Full Text:



Åhman, J., Matuschek, E., & Kahlmeter, G. (2022). Evaluation of ten brands of pre-poured Mueller-Hinton agar plates for

Eucast disc diffusion testing. Clinical Microbiology and Infection, 28(11), 1499.e1-1499.e5. https://doi.org/10.1016/J.CMI.2022.05.030

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/J.JPHA.2015.11.005

Bolukaoto, J. Y., Monyama, C. M., Chukwu, M. O., Lekala, S. M., Nchabeleng, M., Maloba, M. R. B., Mavenyengwa, R. T., Lebelo, S. L., Monokoane, S. T., Tshepuwane, C., & Moyo, S. R. (2015). Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa. BMC Research Notes, 8(1), 6–12. https://doi.org/10.1186/s13104-015-1328-0

Bulotta, S., Celano, M., Lepore, S. M., Montalcini, T., Pujia, A., & Russo, D. (2014). Beneficial effects of the olive oil phenolic components oleuropein and hydroxytyrosol: focus on protection against cardiovascular and metabolic diseases. Journal of Translational Medicine, 12(1). https://doi.org/10.1186/S12967-014-0219-9

de Melo, S. C. C. S., Costa, A. B., da Silva, F. T. R., Silva, N. M. M. G., Tashima, C. M., Cardoso, R. F., de Pádua, R. A. F., Previdelli, I., Carvalho, M. D. de B., & Pelloso, S. M. (2018). Prevalence of Streptococcus agalactiae colonization in pregnant women from the 18th Health Region of Paraná State. Revista Do Instituto de Medicina Tropical de Sao Paulo, 60(November 2017), 2–7. https://doi.org/10.1590/s1678-9946201860002

de Melo, S. C. C. S., Santos, N. C. De S., De Oliveira, M., Scodro, R. B. De L., Cardoso, R. F., Pádua, R. A. F., Silva, F. T. R., Costa, A. B., Carvalho, M. D. De B., & Pelloso, S. M. (2016). Antimicrobial Susceptibility Of Streptococcus Agalactiae Isolated From Pregnant Women. Revista Do Instituto de Medicina Tropical de Sao Paulo, 58(1), 2–5. https://doi.org/10.1590/S1678-9946201658083

do Nascimento, C. S., dos Santos, N. F. B., Ferreira, R. C. C., & Taddei, C. R. (2019). Streptococcus agalactiae in pregnant women in Brazil: prevalence, serotypes, and antibiotic resistance. Brazilian Journal of Microbiology, 50(4), 943–952. https://doi.org/10.1007/S42770-019-00129-8/Metrics

Donadio, G., Mensitieri, F., Santoro, V., Parisi, V., Bellone, M. L., De Tommasi, N., Izzo, V., & Piaz, F. D. (2021). Interactions with microbial proteins driving the antibacterial activity of flavonoids. Pharmaceutics, 13(5), 660.


Farhadi, F., Khameneh, B., Iranshahi, M., & Iranshahy, M. (2019). Antibacterial activity of flavonoids and their structure–activity relationship: An update review. Phytotherapy Research, 33(1), 13–40. https://doi.org/10.1002/PTR.6208

Genovese, C., D’Angeli, F., Di Salvatore, V., Tempera, G., & Nicolosi, D. (2020). Streptococcus agalactiae in pregnant women: serotype and antimicrobial susceptibility patterns over five years in Eastern Sicily (Italy). European Journal of Clinical Microbiology and Infectious Diseases, 39(12), 2387–2396. https://doi.org/10.1007/s10096-020-03992-8

Geromichalou, E. G., & Geromichalos, G. D. (2022). In Silico Approach for the Evaluation of the Potential Antiviral Activity of Extra Virgin Olive Oil (EVOO) Bioactive Constituents Oleuropein and Oleocanthal on Spike Therapeutic Drug Target of SARS-CoV-2. Molecules, 27(21). https://doi.org/10.3390/molecules27217572

Gupta, A., Mumtaz, S., Li, C. H., Hussain, I., & Rotello, V. M. (2019). Combatting antibiotic-resistant bacteria using nanomaterials. Chemical Society Reviews, 48(2), 415–427. https://doi.org/10.1039/C7CS00748E

Jimenez-Lopez, C., Carpena, M., Lourenço-Lopes, C., Gallardo-Gomez, M., M. Lorenzo, J., Barba, F. J., Prieto, M. A., & Simal-Gandara, J. (2020). Bioactive compounds and quality of extra virgin olive oil. Foods, 9(8). https://doi.org/10.3390/foods9081014

Perez-Gavilan, A., de Castro, J. V., Arana, A., Merino, S., Retolaza, A., Alves, S. A., Francone, A., Kehagias, N., Sotomayor-Torres, C. M., Cocina, D., Mortera, R., Crapanzano, S., Pelegrín, C. J., Garrigos, M. C., Jiménez, A., Galindo, B., Araque, M. C., Dykeman, D., Neves, N. M., & Marimón, J. M. (2021). Antibacterial activity testing methods for hydrophobic patterned surfaces. Scientific Reports 2021 11:1, 11(1), 1–10. https://doi.org/10.1038/s41598-021-85995-9

Qabaha, K., Al-Rimawi, F., Qasem, A., & Naser, S. A. (2018). Oleuropein Is Responsible for the Major Anti-Inflammatory Effects of Olive Leaf Extract. Https://Home.Liebertpub.Com/Jmf, 21(3), 302–305. https://doi.org/10.1089/JMF.2017.0070

Raabe, V. N., & Shane, A. L. (2019). Group b streptococcus (streptococcus agalactiae). Gram-Positive Pathogens, 11, 228–238. https://doi.org/10.1128/9781683670131.ch14

Rahardjo, B; Rahmawati, W; Rahasti, A;

Retnaningrum, D.N; Sujuti, H. N. A. R. (2020). Effect of Evoo on Mda, Adma and no Level in Rattus Norvegicus Pre-Eclampsia Model. Indian Journal of Public Health Research & Development, 11(3), 1874–1880. http://medicopublication.com/index.php/ijphrd/article/view/2197

Rahardjo, B., Rahmawati, W., Rahasti, A., Retnaningrum, D. N., Sujuti, H., As, N., & Raras, T. Y. M. (2020). The effect of extra virgin olive oil to decrease HSP-90, TNF-α and ET-1, in pre-eclampsia rat model. Journal of Global Pharma Technology, 12(1).

Savini, V., Marrollo, R., D’Antonio, M., D’Amario, C., Fazii, P., & D’Antonio, D. (2013). Streptococcus agalactiae vaginitis: Nonhemolytic variant on the Liofilchem® Chromatic

StreptoB. International Journal of Clinical and Experimental Pathology, 6(8), 1693–1695.

Shabayek, S. (2022). Streptococcus agalactiae (Group B Streptococcus). Molecular Typing in Bacterial Infections, Volume I: Second Edition, 1(11), 167–189. https://doi.org/10.1007/978-3-030-74018-4_8

Sharma, V. K., Johnson, N., Cizmas, L., McDonald, T. J., & Kim, H. (2016). A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes. Chemosphere, 150, 702–714.

Slavin, Y. N., Asnis, J., Häfeli, U. O., & Bach, H. (2017). Metal nanoparticles: Understanding the mechanisms behind antibacterial activity. Journal of Nanobiotechnology, 15(1), 1–20. https://doi.org/10.1186/S12951-017-0308-Z/FIGURES/1

Summerhill, V., Karagodin, V., Grechko, A., Myasoedova, V., & Orekhov, A. (2018). Vasculoprotective Role of Olive Oil Compounds via Modulation of Oxidative Stress in Atherosclerosis. Frontiers in Cardiovascular Medicine, 5(December), 1–10.

Tejada, S., Pinya, S., Mar Bibiloni, M. del, Tur, J. A., Pons, A., & Sureda, A. (2016). Cardioprotective Effects of the Polyphenol Hydroxytyrosol from Olive Oil. Current Drug Targets, 18(13). https://doi.org/10.2174/1389450117666161005150650

V.Syafriana, R.D.Rentiana, & Poeloengan, dan M. (2016). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Dan Biji Pepaya (Caricapapaya L.) Terhadap Streptococcus Agalactiae. Fakultas Farmasi, Institut Sains Dan Teknologi Nasional, Jakarta, 9(2), 19–22.

Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Current Medicinal Chemistry, 22(1), 132-149(18).

Abstract - Print this article - Indexing metadata - How to cite item - Finding References - Email this article (Login required) - Email the author (Login required)


  • There are currently no refbacks.