Putu Oky Ari Tania, I Kadek Angga Dwi Cahyadi


A high-fat diet leads to increasing intake of fatty acid in liver, making it susceptible to lipid peroxidation and free radical release. Malondialdehyde is a common biomarker for lipid peroxidation. Increasing free radicals should be balanced with the antioxidants in order to prevent oxidative stress. This study aim was to measured the effectiveness of bay leaf extract (Eugenia polyantha) in lowering MDA levels in hyperlipidemia rats. This study was an experimental study using 20 male rats divided into 4 groups. All rats were induced hyperlipidemia with high-fat diet for 12 days. KP was sacrificed in the next day after the feeding of high fat. KN administered by aquades 2 ml / day (peroral / po), P1 administered by bay leaves extract 1.08 g / day (p.0) and P2 received simvastatin treatment dose 0,0018 g / 150 g BB / day (p. 0). The results showed MDA levels in KP, KN, P1 and P2 were respectively 7.79; 7.07; 6.49; and 7.21 nmol / ml. One way ANOVA test showed p value was 0,073 (<0,05) so there was no effect of effectiveness of bay leaf in lowering MDA level compared to simvastatin and aquades.


bay leaf, high-fat diet, MDA

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Birben, et al. (2012). Oxidative stress and antioxidant defense. WAO journal 2012: 1-19

Estradella, et al. (2013). Lipotoxicity : Effects of dietary saturated and transfatty acids. Mediators of Inflammation 2013:1-13.

Fernandez and West. (2005). Mechanisms by which dietary fatty acids modulate plasma lipids. Americans Society for Nutritional Sciences. 2075-2078.

Hassan, et al. (2011). Improvement of lipid profile and antioxidant of hypercholesterolemia albino rats by polysaccharides extracted from the green alga Ulva lactusa Linnaeus. Saudi Journal of Biological Sciences 18:333-340.

Harini and Astirin (2009). Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Bioscience 1(2):53-58.

Narita (2015). Bay leaf in dislipidemia therapy. J. Majority 4(4):64-69.

Indrayana (2008). Efek antioksidan ekstrak etanol 70% daun salam (Syzygium polyanthum) pada serum darah tikus putih jantan galur wistar yang diinduksi karbon tetraklorida (CCl4). Skripsi, Fakultas Farmasi Universitas Muhammadiyah Surakarta.

Lelono, et al. (2009). in Vitro antioxidative activities and polyphenol content of Eugenia polyantha Wight grown in Indonesia. Pakistan Journal of Biological Sciences. 2009; 12(24):1564-1570.

Noeman, et al. (Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetology & Metabolic Syndrome. 2011; 3(17);1-8.

Olorunnisola SO, Bradley G, and Afolayan AJ. Protective effect of T. violacea rhixome extract against hypercholesterolemia-induced oxidative stress in Wistar rats. Molecules. 2012; 17:6033-6045.

Safriani N, Arpi N, and Erfiza NM. Potency of curry (Murayya koeniigi) and salam (Eugenia polyantha) leaves as natural antioxidant sources. Pakistan Journal of Nutrition. 2015; 14(3):131-135.

Sanchez A, Calpena AC, and Clares B. Evaluating the oxidative stress in inflammation: role of melatonin. Int. J. Mlo. Sci. 2015; 16:16981-17004.

Suryadinata RV, Wirjatmadi B, and Adriani M. Efektivitas penurunan malondialdehyde dengan kombinasi suplemen antioksidan superoxide dismutase melon dan gliadin akibat paparan rokok. Global Medical and Health Communication. 2017; 5(2):79-83.

Siregar RNI. The effect of Eugenia polyantha extract on LDL cholesterol. J. Majority. 2015; 4(5):85-92.

Setiawati A, and Nafrialdi. Farmakologi dan Terapi. UI. 2007; edisi kelima.

Tirzitis G, and Bortosz G. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochimica Polonica. 2010; 57(1):132-142.

Wu P, Zhang F, Dai Y, Han L, and Chen S. Serum TNFα, GTH and MDA of high-fat diet-induced obesity and obesity resistant rats. Saudi Pharmaceutical Journal. 2016; 24;333-336.

Yang R, Shi Y, Hao G, Li W, and Le G. Increasing oxidative tres with progressive hyperlipidemia in human: relation between malondialdehyde and atherogenic index. J. Clin. Biochem. Nutr. 2008; 43:154-158.

Zimmermann AM, Rivero AC, Bezerra AS, Ruviaro AR, Novack MME, and Nornberg JL. Antioxidant potential of barley extract in rats subjected to a high-fat diet. Food Sciene and Technology. 2013; 33(1);167-171.

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