EFEK PAPARAN ALGINAT DALAM PANGAN TERHADAP KADAR PROTEIN TOTAL, ALBUMIN DAN GLOBULIN DARAH

Pramono Sasongko, Wahyu Mushollaeni

Abstract

Clinical analysis to determined the toxicity of new material could be done by biochemical analysis of wistar rat blood serum such as blood protein, albumin, and globulin content. In case of decreasing of those biochemical analysis indicated the reduction of liver function in metabolism process. Hence, this study were conducted in order to determined toxicity effect of alginate presence extracted from brown seaweed Turbinaria sp.due to the blood protein, albumin, and globulin content of wistar rat blood serum. The study were conducted by Complete Randomized Design (CRD) with alginate concentration treatment which were 0.5%; 0.75%; 1%, and negative control. The results were shown that the treatment up to 1% of alginate concentration were not provide any toxicity effect in liver organ. These result was concluded due to the blood protein, albumin, and globulin content were presently still in a safe level.

Keywords

toxicity analysis, blood total protein, albumin, globulin, alginate

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References

Agbaje, E. O., Adeneye, A. A., and Daramola, A. O. 2009. Biochemical and toxicological studies of aqueous extract of Syzigium aromaticum (L.) Merr. & Perry (Myrtaceae) in rodents. African Journal of Traditional, Complementary, and Alternative Medicines 6(3): 241-254.

Aprilia, R. P., Pramana, A. W. M. and Oktavianie, D. A. A. P. 2012. Specificity and sensitivity of thyroglobulin and serum protein profile of autoimmune thyroiditis model rat (Rattus norvegicus) induced by sodium iodide. Diakses: July 5, 2017.https://fkh.ub.ac.id/wp-content/uploads/2012/10/10.-Risky-Pamwindya.pdf. Crandall, J. P. 2007. Diabetus mellitus (DM). Available from: http://www.merck. /mmpe/sec12/ch158/ch158b.html Dianitami, R. 2009. Efek Rumput Laut Eucheuma sp. terhadap Kadar Glukosa Darah dan Jumlah Trombosit Tikus Wistar yang Diinduksi Aloksan. [cited 2013 August 05]. Available from: http://eprints.undip.ac.id/7761/1/ratna_dia nitami.pdf Giknis, M. and Clifford, C. 2008. Clinical laboratory parameters for Crl:WI (Han). Charles River. 1-18 p. Gitawati, R. 1995. Radikal Bebas – Sifat dan Peran dalam Menimbulkan Kerusakan/Kematian Sel. Cermin Dunia Kedokteran, 102 : 33. Jorum, O. H., Piero, N. M. and Machocho, A. K. 2016. Haematological effects of dichloromethane-methanolic leaf extracts of Carissa edulis (Forssk.) vahl in normal rat models. Journal Hematology & Thromboembolic Diseases 4(1): 2-8. Kanu, K. C., Solomon, N. I. and Atiata, O. 2016. Haematological, biochemical and antioxidant changes in wistar rats exposed to dichlorvos based insecticide formulation used in Southeast Nigeria. Toxics 4(28): 2-8.

Kemasari, P., Sangeetha S, and Venkatalakshmi P. Antihyperglycemic activity of Mangifera indica Linn. in alloxan induced diabetic rats. J Chem Pharm Res. 2011;3(5):653-9. Mirshafiey, A., Cuzzocrea, S., Rehm, B., Mazzon, E., Saadat, F. and Sotoude, M. 2005. Sodium Alginate as A Novel Therapeutic Option in Experimental Colitis . Scand J Immunol 61 : 435 – 441 Murray, K., Graner, D., Mayes, P. dan Rodwel, V. 2003. Biokimia. Harper. Edisi 25. EGC. Jakarta. l734-1748 pp. Mushollaeni, W., Supartini, N. and Sriwaningsih, E. R. 2014. Toxicity Test of Alginate from Sargassum and Padina on the Liver of Mice. Food and Public Health 4(4): 204-208. Razavi, A., Khodadadi, A., Eslami, M. B., Eshraghi, S. and Mirshafiey, A. 2008. Therapeutic Effect of Sodium Alginate in Experimental Chronic Ulcerative Colitis . Iran J Allergy Asthma Immunol 7: 13 – 18. Sammad, F. H. A., Athiro, N. A. S. and Santoso, H. 2017. Injection of Scurrula atropurpurea (Bl) Dans metanolic extract in sub-chronic to total protein and albumin of female mice. Bioscience-Tropic 2(2): 49-54. Schroder, S., Palinski, W. and Schmid-Schonbein, G. W. 1991. Activated Monocytes and Granulocytes, Capillary Nonperfusion, and Neovascularization in Diabetic Retinopathy. American Journal of Pathology, 1991 July; 139(1) : 81 – 100.

Smith, J. B. dan Mangkoewudjojo. 1998. Pemeliharaan, pembiakan dan penggunaan hewan coba di daerah tropis.

Suptijah, P. 2009. Rumput Laut: Prospek dan Tantangannya. [cited 2013 August 06]. Available from: http://tumoutou.net/702_04212/pipih_suptijah.htm Sutedjo, S. K. M. 2007. Mengenal penyakit melalui hasil pemeriksaan laboratorium. Amara Books. Yogyakarta. Wibowo, T. A. A. 2009. Efek Diet Rumput Laut Eucheuma sp. terhadap Jumlah Monosit Tikus Wistar yang disuntik Aloksan. [cited 2013 August 05]. Available from: http://eprints.undip.ac.id/7768/ Wikanta, T., Damayanti, R. dan Rahayu, L. 2008. Pengaruh Pemberian Κ-Karagenan dan Ί-Karagenan terhadap Penurunan Kadar Glukosa Darah Tikus Hiperglikemia. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 3(2): 131-138.

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