AKTIVITAS ANTIOKSIDAN EKTRAK METANOL DAN AIR TEH HERBAL POKAK ( Solanum torvum ) TERHADAP ANTIBAKTERI PATOGEN

Nunuk Helilusiatiningsih

Abstract

Pokak (Solanum torvum) can thrive in Indonesia and had high phytochemical and antioxidant compounds. The aim of this study was to analyze the effect of antioxidant compounds on herbal tea products, namely methanol extract of pokak herbal tea and water extract of pokak herbal tea on the inhibition of the growth of pathogenic bacteria. The herbal tea processing method uses surface response. The measured parameters were the total phenol content, tannins, flavonoids, and antioxidant activity (% DPPH Imbibition) and test for pathogenic bacteria inhibition zone using the disc method. The results showed that the best treatment was pokak green tea water extract containing 93.81% antioxidant activity (% DPPH inhibition), total phenol 55.20 mgGAE/g, tannin content 1.70 mgTAE/g, flavonoid levels 1.78 mgQAE/g. Analysis of pokak green tea water extract at a concentration of 100% had an inhibitory power against pathogenic bacteria E coli 20 mm, 20 mm MRSA, 20 ml Salmonella typhi, 19 mm Streptococcus pneumonia. Pokak green tea water extract can be used as a drink that functions for health in inhibiting the growth of pathogenic bacteria.

Keywords

Antioxidants; antibacteria; disc method; pathogenic bacteria; pokak.

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References

Ahmed and Bahar.2007. Chemistry 0f Natural Products. New Delhi: Departemen of Pharmaceutical Chemistry Faculty of Science Jamia Hamdard.

Amador Perez MC., Ocetero V., Munoz,

Castaneda JM., Garcia, Esquinca

AR, and Gonzales, 2007. Alkaloids in Solanum torvum Sw. International Journal Of Experimental Botany. Miguel Raggio and Nora Moro De Raggio

Ashok PK, Upadhyaya K. 2012. Tannins

are Astringen. Journal of Pharmacognosy and Phytochemistry, 1(3): 45-50.

Chah KF., Muko KN., Oboegbulem SI., 1999. Antimicrobial Activity of Methanolic Extract of Solanum torvum Fruit. Short report. Fitoterapia. Nigeria.

Darsana. I, Besung, I. dan Mahatmi, H. 2012. Potensi Daun Binahong (Anredera cordifolia (Tenore) Steenis Dalam Menghambat Pertumbuhan Bakteri E coli secara invitro. Indonesia Medicus Veterinus.

Govindaraju. K, Tamilselvan S, Kiruthiga V, and Singaravelu G, 2010. Biogenic Silver Nanoparticles by Solonum torvum And Their Promising Antimicrobial Activity. Jurnal of Biopesticides 3(1 Special Issue) 394-399

Harborne, J. B. 2006. Metode Fitokimia. Edisi ke – 2. Bandung: ITB.

Hendra R1, Ahmad S, Sukari A, Shukor MY, and Oskoueian E., 2011. Flavonoid analyses and antimicrobia activity of various parts of Phaleria macrocarpa (Scheff)Boerl fruit Int. J. Mol Sci: 12: 3422-3431.

Jaiswal BS., 2011. Solanum torvum: A Review of Its Traditional Uses.

PhytoChemistry And Pharmacology. International Jurnal Of Pharma and Bio Sciences, SOS in Pharmaceutical Sciences. Jiwaji University. Cwalior. India

Karmakal K., Islam MDA., Chanda SA., Tuhin TI., Muslim T., and Rahman Md A., 2015. Secondary Metabolites from The Fruits of Solanum torfum SW. Journal of Pharmacognosy and Phytechemistry. 4(1): 160-163. Departement Of Chemistry University of Dhaka.Banglades.

Li J., Zhang Lu, Huang C., Guo F., and Li Yiming, 2014. Five New Cytoxic Steroidal Glycosides from The Fruit of Solanun torvum. Fitoterapia. China.

Levinson, Jawetz. 2000. Medical Microbiology and Imunology: Examinitation and Board Review, 6th ED., McGraw- Hil, Singapore, p.87.

Lewis, T. 2009. Steroid Interference with antimikrobial activity of polyene antibiotics. Appl. Microbiol. 14: 865-869.

Marinova, D., F., Ribarova and M. Atanasova. 2005. Total phenolics and total flavonoids in Bulgarian fruit and vegetables. J. Univ. Chem. Technol. Metallurgy. 40:255-260

Madduluri S, Rao K.B, and Sitaram B. 2013. In Vitro Evaluationof Antibacterial Activity of Five Indegenous Plants Exstrac Against Five Bacterial Pathogegens of Human. International Journal of Pharmacy and Pharmaceutical Sciences: 5(4): 679-684.

Nuria, Maulita Cut, faizaitun, Arvin, dan Sumantri. 2009. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus Aureus Atcc 2592, E coli Atcc25922, dan Salmonella Typhi Atcc 1408. Mediagro: 5(2): 26-37.

Meda, A., Lamien, CE., Romito, M., Millogo, J., Nacoulma, OG. 2005. Deternnition of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry. Vol.91 (571-577). Elsevier.

Rammohan M., And Reddy C., Srinivas, 2010. Anti- Inflamatory Activity of Seed and Fruit Wall Extract of Solanum torvum. Journal Fordrugsand Medicines, Hygeia, J.D.Med.vol.2(2), 2010, 54-58.

Razak A., Djamal A., Dan Revilla G., 2013. Uji Daya Hambat Air Perasan Jeruk Nipis (Citrus aurantifolia, S.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Kesehatan Andalas. FK Universitas Andalas. Padang.

Palczar, J.M dan Chan, E.C.S. 1988. Dasar- Dasar Mikrobiologi 2. Jakarta. Penerbit UI Press.

Sari, F. P. dan S.M. Sari. 2011. Ekstraksi Zat Aktif Antimikroba dari Tanaman Yodium (Jatropha multifida Linn) sebagai Bahan Baku Alternatif Antibiotik Alami. Semarang Fakultas Tehnik Universitas Diponegara.

Soutan KO, Oyekunle MA, Aiyelaagbe OO, Fafunso MA. 2006. Evaluation of The Antimicrobial Activity of Saponin Extract of Sorghum Bicolor L. Moench.African Jurnal of Biotechnology, 5(23): 2405- 2407.

Tim Mikrobiologi FK Unibraw, 2003. Bakteriologi Medik Edisi 1. Malang: Bayumedia Publishing.

Wannasiri S, Chansakaow S, and Sireeratawong S, 2017. Effects of Solanum torvum Fruit Water Extract on Hyperlipidemia and Sex Hormones In High-Fat Fed Male Rats. Asian Pacific Jurnal of Tropical Biomedicine. Thailand:7(5). 401-405.

Yuan-Lu Y., Guang LJ., and Yi K., 2011. Chemical contituents from Solanum torvum. Chinese Journal of Natural Medicines. 9(1): 0030- 0032.China.

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