Kombinasi Glucomannan Hydrolysates (Gmh) Dan Antibiotik Metronidazole Berpengaruh Terhadap Kadar Sitokin Il-23 Pada Bacterial Vaginosis  Wanita Usia Subur

Anik Sri Purwanti; Sumarno Sumarno; Bambang Rahardjo; Sri Winasih; Sri Poeranto

doi:10.33366/jc.v7i1.1105

Kombinasi Glucomannan Hydrolysates (Gmh) Dan Antibiotik Metronidazole Berpengaruh Terhadap Kadar Sitokin Il-23 Pada Bacterial Vaginosis Wanita Usia Subur

Anik Sri Purwanti, Sumarno Sumarno, Bambang Rahardjo, Sri Winasih, Sri Poeranto

Abstract

Woman’s reproductive tract often has problems, especially in women of childbearing age. One of them is Bacterial Vaginosis is a clinical condition that occurs a lot. BV infection is a polymicrobial infection caused by a decrease in the amount of Lactobaclilus with anaerobic bacteria that increases excessively. Alternative treatment for BV is given by using the results hydrolysates of konjac plant extracted in dosage forms Glucomannan Hydrolysates (GMH) containing glucose and mannose prebiotic which can support the growth development of Lactobacillus in the vaginal mucosa. This study was to determine the effect of Glucomannan Hydrolisates (GMH) + Metronidazole on levels of IL 23 cytokines in BV of reproductive age women. The method of this study used the True Experimental design with the type of research The randomized pretest-posttest was in vivo. The subjects selected in this study design used Randomized techniques. A sample of 7 people WUS with BV given GMH 300mg + Metronidazole 1000 mg for 9 days of use, To assess the levels of cytokine IL 23 using the ELISA method. Data analysis using a ratio scale was analyzed using the metric statistical test, normality test using Shapiro-Wilk test and comparative test using Repeated Measure ANOVA. The overall results of the analysis showed that the combination of GMH and Antibiotics Metronidazole could significantly increase the levels of cytokine IL 23 compared to other therapies to treat bacterial vaginosis in women of childbearing age

Keywords

Bacterial Vaginosis (BV); Glucomannan Hydrolysates (GMH); IL-23 levels

Full Text:

PDF

References

Al-Ghazzewi, F., Elamir, A., Tester, R., & Elzagoze, A. (2015). Effect of depolymerised konjac glucomannan on wound healing. Bioactive Carbohydrates and Dietary Fibre, 5(2), 125–128. https://doi.org/10.1016/j.bcdf.2015.03.003

CDC. (2017). Bacterial Vaginosis - CDC Fact Sheet. Centers for Disease Control and Prevention, 5–6. https://doi.org/10.1016/j.carbpol.2013.10.092

Fethers K, Twin J, Fairley Ck, Fowkes Fj, Garland Sm, Fehler G, E. Al. (2012). Bacterial Vaginosis (BV) Candidate Bacteria: Associations With BV And Behavioural Practices In Sexually- Experienced And Inexperienced Women. Plos One, 7(2), E30633.

Guaschino S, De Santo D, D. S. F. (2002). New perspectives in antibiotic prophylaxis for obstetric and gynaecological surgery. New Perspectives in Antibiotic Prophylaxis for Obstetric and Gynaecological Surgery, 50, S13–S16.

Hay, P. (2014). Bacterial vaginosis. Medicine (United Kingdom), 42(7), 359–363. https://doi.org/10.1016/j.mpmed.2014.04.011

J, W. (2004). Managing recurrent bacterial vaginosis. Sex Transm Infect, 80, 8–11.

Leung, S., Liu, X., Fang, L., Chen, X., Guo, T., & Zhang, J. (2010). The cytokine milieu in the interplay of pathogenic Th1/Th17 cells and regulatory T cells in autoimmune disease. Cellular and Molecular Immunology, 7(3), 182–189. https://doi.org/10.1038/cmi.2010.22

Machado, D., Castro, J., Palmeira-de-Oliveira, A., Martinez-de-Oliveira, J., & Cerca, N. (2016). Bacterial vaginosis biofilms: Challenges to current therapies and emerging solutions. Frontiers in Microbiology, 6(JAN). https://doi.org/10.3389/fmicb.2015.01528

Ness RB, Soper DE, Holley RL, et al. (2001). Douching and endometritis: results from the PID evaluation and clinical health (PEACH) study. Sex Transm Disease. Douching and Endometritis: Results from the PID Evaluation and Clinical Health (PEACH) Study. Sex Transm Disease, 28, 240–245.

Patterson, J. L., Stull-Lane, A., Girerd, P. H., & Jefferson, K. K. (2010). Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes. Microbiology, 156(2), 392–399. https://doi.org/10.1099/mic.0.034280-0

Sutherland, A. ; R. T. ; F. A.-G. ; E. M. ; M. C. (2008). Glucomannan hydrolysate (GMH) inhibition of Candida albicans growth in the presence of Lactobacillus and Lactococcus species. Microbial Ecology in Health and Disease, 20:3, 127 — 134. Retrieved from http://dx.doi.org/10.1080/08910600802355726

Tester, R. F., & Al-Ghazzewi, F. H. (2013). Mannans and health, with a special focus on glucomannans. Food Research International, 50(1), 384–391. https://doi.org/10.1016/j.foodres.2012.10.037

Thurman, A. R., Kimble, T., Herold, B., Mesquita, P. M. M., Fichorova, R. N., Dawood, H. Y., Doncel, G. (2015). Bacterial Vaginosis and Subclinical Markers of Genital Tract Inflammation and Mucosal Immunity. AIDS Research and Human Retroviruses, 31(11), 1139–1152. https://doi.org/10.1089/aid.2015.0006

Workowski, K., & Levine, W. (2002). Sexually transmitted diseases treatment guidelines 2002. Centers for Disease Control and Prevention. MMWR Recomm Rep, 51(RR-6), 1–78. https://doi.org/10.1152/jn.00500.2012

Zheng, Yan., Dimitry M., Danilenko., Patricia Valdez., Ian Kasman., Jeffrey Eastham-Anderson., J. W. & W. O. (2007). Interleukin-22, a TH17 cytokine, mediates IL-23-induced dermal inflammation and acanthosis, Vol 445|8.

DOI: https://doi.org/10.33366/jc.v7i1.1105

Abstract - Print this article - Indexing metadata - How to cite item - Finding References - Email this article (Login required) - Email the author (Login required)